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KMID : 0861020120270010065
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Korea Journal of Herbology
2012 Volume.27 No. 1 p.65 ~ p.71
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Nelumbinis Folium Methanol Extract Regulates Inducible Nitric Oxide Synthase Signaling Pathways in Mouse Peritoneal Macrophages
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Lee Si-Eun
Lee Mi-Hwa
Choi Ho-Young
Sohn Nak-Won
Kang Hee
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Abstract
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Objective: Nelumbinis Folium (NF) is used for diarrhea, headache and dizziness in traditional medicine. In this
paper, we examined the anti-inflammatory effects of the methanol extract of NF in mouse macrophages.
Methods: Peritoneal macrophages from thioglycollate medium-injected mice were cultured and stimulated with
lipopolysaccharide(LPS) or LPS/interferon(IFN)-γ for viability assay, cytokine measurement and Western
blotting.
Results: NF methanol extract suppressed the levels of nitric oxide (NO) through reduction of inducible NO
synthase in a concentration-dependent manner. The extract reduced LPS/IFN-γ-stimulated STAT1
phosphorylation and LPS-induced IκBα degradation through inhibition of IκBα kinase activation. The extract
also inhibited p38, JNK/SAPK and ERK1/2 activation.
Conclusions: Our findings suggested that NF has anti-inflammatory activity, and have a potential for
therapeutic application. Further research is required to investigate its anti-inflammatory active compounds.
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KEYWORD
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Nelumbinis Folium, inflammation, macrophages, nitric oxide synthase, signaling
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